Cryo-Correlative Workflow Specialist & Microscopist

Key Information Hours per week: 35 hours per week (Full Time) Job Details We are seeking to appoint a Senior Scientific Officer to work collaboratively within both the Light and Electron Microscopy Facilities at the Institute of Cancer Research London. You would be joining an enthusiastic team of microscopists in supporting/implementing world class experiments and developing cryo-correlative workflows at the ICR.  This post is a collaboration between the Light Microscopy Facility (Core Research Facilities) and the Electron Microscopy Facility (Structural Biology) to create cryo-correlative light and electron microscopy (CLEM) workflows for in situ targeting (confocal and FIB-SEM techniques) of specific molecules and proteins and ultra-high resolution 3D Cryo-Electron Tomography (Cryo-ET) of these targets in cancer. The objective of this post is to work under the direction and collaboration of the associated Facility Managers to operate and maintain the associated equipment in these workflows, and provide training to researchers at the institute. This post builds on our recent success in obtaining funding, and purchasing equipment, that facilitates state-of-the-art in situ Structural Biology workflows for cryo-ET in cells and tissues across scale.  Work will include: performing regular quality control and maintenance of the microscopes / cryo preparation equipment; advising facility users on the best techniques and providing applications advice; giving detailed one-to-one training sessions to our researchers on how to use the advanced instrumentation and workflows for their studies; and designing detailed image analysis protocols. The post-holder will also be expected to participate in giving regular training courses to members of the institute in in situ structural biology and image analysis. Key Requirements The successful candidate will have experience in multimodal imaging techniques in light/electron microscopy and demonstrate the following key skills: Vitrification of cells and tissues via plunge & high pressure freezing for cryo light and electron microscopy imaging. Proven experience in operating advanced light and electron microscopy equipment (e.g. SEM, TEM, fluorescence/confocal microscopy). Experience in sub-tomographic averaging, electron tomographic reconstruction, correlation, and segmentation using open source and commercial software (e.g. IMOD, Amira). Ability to critically judge the quality/feasibility of cryo-ET and cryo-CLEM projects. Demonstrable ability to support researchers from different teams in their research projects through training and technical supervision/guidance. Demonstrable ability to interact with all users of the shared research facility, including PhD students, postdoctoral fellows, research staff and collaborators. Department/Directorate Information: This post is a collaboration between the Light Microscopy Facility (Core Research Facilities) and the Electron Microscopy Facility (Structural Biology). At the ICR we aim to defeat cancer through scientific excellence, innovation and partnership. These principles also underpin our approach to scientific infrastructure, which is among the very best of any research centre in the UK.. About The Institute of Cancer Research Why work for us? As a member of staff, you'll have exclusive access to a range of staff benefits. The ICR is committed to supporting overseas applicants applying for roles, please click here to find out further information. We look forward to receiving applications from all candidates, wherever in the world they are currently based. However, we particularly welcome British applicants from black and ethnic minority backgrounds, as they are under-represented within the ICR and nationwide in STEM roles.