Research Associate

Job description Applications are invited for the position of Post-Doctoral Research Associate in the Department of Diabetes at King’s College London, working in the laboratories of Dr. Gavin Bewick. This is an MRC funded project entitled Decoding the Obese Duodenum. Its goal is to understand how the duodenum, changes in response to obesity and how these changes may exacerbate metabolic diseases. We will do this by multiomics mapping the human obese duodenum, establish how key pathological pathways disturb gut function and whole-body physiology using organoid technology and animal models and determine the therapeutic potential of restoring duodenal function. The successful candidate will work as part of team and be responsible for carrying out the multiomic mapping and gut organoid work. They will have a PhD in a relevant biomedical sciences subject and have expertise in in vitro assessment of cell function and single cell transcriptomics. Prior experience of gut stem cell biology and gut organoid culture would be a distinct advantage.  This post will be offered on a fixed-term contract until 28 February 2027. This is a full-time post - 100% full time equivalent. Key responsibilities Management of the research project on a day-to-day basis  Maintaining lines of communication with Dr. Gavin Bewick Integrating within the wider Department of Diabetes at King’s College London. Supervision of MSc/BSc/visiting students where required. Presenting work at national and international conferences. The above list of responsibilities may not be exhaustive, and the post holder will be required to undertake such tasks and responsibilities as may reasonably be expected within the scope and grading of the post. Skills, knowledge, and experience Essential criteria  Expertise in general molecular cell biology techniques such as cell/tissue culture, qRT-PCR, immunoblotting, immunoassays, (immuno)histochemistry.  Experience with genetic manipulation eg. Crispr, PiggyBAC Experience of single-cell transcriptomics. Willing and able to work in a team. Ability to keep clear and well-organised laboratory notes and records. Ability to plan and prioritise workload. Good communication (both written and verbal) and presentation skills. Self-motivated, ability to work independently.  Desirable criteria Experience with culturing mouse or human gut organoids. Experience of in vitro assessment of cell function including viability, apoptosis, proliferation, ATP generation, secretory capacity, FACS. Further information This post is subject to Occupational Health clearance. Closing date: 12 February 2024